Tomato cultivation in the state of Sinaloa represents a third of the production in Mexico. Technological innovation techniques have now been incorporated into its production, moving from soil cultivation to stick cultivation and under controlled conditions in greenhouses; offering products with low or no agrochemical residues, seeking to reverse contamination problems in addition to these practices increasing the value of the product. The objective of this research was to develop an efficient and reproducible system for the in vitro propagation of tomato plants (Solanum lycopersicum) without the use of exogenous growth regulators, using dry mature seeds of six tomato varieties (hybrid brigade, hybrid maya; sun 6200; sun 6366; calista; DRD 8561). Under sterile conditions, the seeds were cultivated in a medium with salts (MS) Murashige and Skoog [1], without growth regulators, under controlled conditions (photoperiod of 16 h.day-1) at 25 ± 20oC. After five weeks of incubation, vitro plants were obtained, which presented a positive response to the aforementioned conditions, obtaining stems of good size, presence of leaf tissue, and abundant root growth, showing efficient and rapid tissue reproduction and easy handling. This work presents an efficient plant regeneration system with the advantages of not using exogenous growth regulators and easy handling from adventitious shoots, demonstrating that the micropropagation technique helps to produce more plants in less time