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Environmental Sciences and Ecology: Current Research


In Vitro Propagation of A Cuban Arbuscular Mycorrhizal Fungal Strain

Research Article
Volume 3 - Issue 5 | Article DOI : 10.54026/ESECR/1063

Sussy Saymara Perera Garcia1 , Kalyanne Fernández Suarez*1, Eduardo José Perez Ortega1 , Yonaisy Mujica Perez1 , Renee Perez Perez2 and Yakelin Rodríguez Yon1

1Instituto Nacional de Ciencias Agrícolas, Department of Biofertilizers and Plant Nutrition, Cuba
2Instituto Nacional de Ciencias Agrícolas, Department of Crops Physiology and Biochemestry, Cuba

Corresponding Authors

Kalyanne Fernández Suarez, Instituto Nacional de Ciencias Agrícolas, Department of Biofertilizers and Plant Nutrition, carretera San José-Tapaste km 3 ½, 32 700 San José de las Lajas, Mayabeque, Cuba

Keywords

Arbuscular Mycorrhizae; In Vitro Culture; MSR Medium

ABSTRACT

In vitro root cultivation techniques based on modified root systems are often used in studies on Arbuscular Mycorrhizal Fungi (AMF). It is a simplified but powerful tool to investigate AMF root colonization and development of the extraradical mycelium. The aim of this study was to establish and characterize the in vitro culture of a Cuban strain of Rhizophagus irregularis (INCAM 11) by using transformed chicory roots. For that, superficially disinfected propagules of R. irregularis were co-culture with the hairy transformed chicory roots on Modified Strullu and Romand (MSR) medium during five months. Spore germination was observed 3-5 days after surface disinfection. The first contact between AMF hyphae and roots occurred 1-3 days after germination and a significant production of extensive extraradical mycelium was observed. New spore formation started within 21-25 days. After 5 months, 2000 spores could be observed per plate which were able to germinate, colonize, establish and reproduce again spores when associated to young transformed roots of chicory. The most frequent associated microorganism to the in vitro culture of INCAM 11 was isolated and identified as Paenibacillus sp.