Canine Distemper Virus (CDV) is a virus that has several potential hosts, all carnivores, which can spread to wild animals, increasing their predisposition in winter. It has a high prevalence and is highly contagious, being one of the main causes of death in domestic and wild canids. Although it is a monotypic virus, that is, with little genetic variability, the H gene has high genetic variation, which would produce different strains of the virus, some more virulent than others and with different tropism. It is because of this variability that patients get sick anyway, since the eightfold vaccine protects against a specific strain of the virus, America 1. So any carnivore susceptible to the virus that faces a strain against which it was not vaccinated has as many chances of getting sick like an animal that was simply never vaccinated against this viral disease. For this reason, it is essential to know which are the lineages of the virus present in the country and for that a diagnostic technique is necessary that not only tells whether the virus is or is not Canine Distemper, but also reveals whether it is one of the two lineages that already exist. knows that they are present or there are others. For this, it is necessary to carry out a multiple RT-PCR protocol, detecting the N gene, which is the least variable, and in parallel the H gene (America 1 and European lineage) to perform a phylogenetic analysis based on official and known sequences of the virus. Thus, the general objective was the use a genomic database in conjunction with the in silico design of primers for the generation of an RT-PCR protocol capable of detecting canine distemper virus and the genotype involved, obtain sequences from GenBank of the CDV genome for gene N, America 1 and European, alignment of sequences with the Clustal W program and generation of consensus sequences and design of rimers using the OligoPerfect program.