Introduction: In a recent publication, we described the production method for Non-Thermal Plasma (NTP) without added external energy. Bulk water contains 12-19% free water molecules. Once filtered through glass into a sealed confined jar, free water molecules reacted to produce an new form of NTP, a gas/water combination, Hybrid-Plasma (HP). This report describes a second production method for HP. Methods: We added 800mL distilled water to a 4000mL acrylic cylinder. Inside the cylinder, an uncovered 500mL collection jar equipped with an ion counter and hygrometer was placed on a platform above the water line. When the acrylic cylinder was sealed with a cover, it formed the confined chamber similar to the water through glass method. Results: After 24 hours, maximum readings were registered on the ion counter and hygrometer inside the open jar confined in the chamber. The uncovered collection jar was sealed with a plastic cover and placed on a shelf for observation (n=6). Initially, the ion counters (n=6) registered a mean ion count of 1443 and a mean hygrometer reading of 93%; both readings gradually declined until day 6. Discussion: We produced HP by a second method with the same properties as HP produced by the original water-throughglass method. Analysis of the HP indicated the presence of Hydroxide/OH- and H2 O2 /Hydrogen Peroxide. Conclusions: We discovered how to separate highly kinetic free water molecules in a confined chamber to produce stable NTP. Hybrid plasma was accumulated, analyzed and used in experiments to determine the effects it has on living systems.