Anthropogenic stresses caused by pesticides use and petroleum-derived pollutants from oil spills are widespread in agroecosystems, yet their direct effects on soil fungi remain poorly understood. This mini-review examines the responses of root-associated Dark Septate Endophytes (DSE) to these contaminants based on experimental evidence from isolated strains. Results show highly variable, strain-dependent responses, ranging from strong growth inhibition to metabolic adaptation and contaminant utilization. Pesticides generally exert more pronounced inhibitory effects, particularly at high concentrations, whereas some fungi display the capacity to degrade both pesticides and hydrocarbons. These findings highlight the functional plasticity of DSE fungi and suggest that anthropogenic stressors can alter fungal activity promoting effects on plant-fungus interactions and ecosystem processes. The adaptive responses of these fungi may play a key role in agroecosystem resilience under increasing environmental pressure.