Pesticides are widely used to improve crop production; however, their impact on human health remains a concern. This study aimed to assess the level of knowledge about pesticide use, identify symptoms after exposure, and evaluate biosecurity measures among farmers in Miraflores. A descriptive, cross-sectional study was conducted in 2025 with 50 participants selected from a population of 4,118 inhabitants. Data was collected through a structured survey. Results showed that 85% of participants had some level of education, but only 35% used complete protective equipment. The most common symptoms reported were headache (60%) and dizziness (30%). A significant proportion of farmers used incomplete or no protection. In conclusion, despite having basic education, farmers lack adequate training in pesticide use, which increases the risk of health problems. Strengthening education and promoting proper biosecurity measures are essential to reduce exposure risks.