Multipotent mesenchymal stromal cells (MSCs) derived from adipose tissue have been increasingly studied due their lessinvasive extraction surgery, self-renew and differentiation potential. The literature has not been reported the comparison of different sources of MSCs derived from distinct adipose tissues and the use of electrical stimulation (ES) for tooth and bone tissue regeneration. The present study evaluated in vitro cell viability by MTT, cell proliferation by CCK-8 and the osteogenic differentiation by alizarin red-S staining of MSCs from Wistar rats adipose tissue, abdominal (ABD) and inguinal (ING), submitted to ES therapy. ES was applied during 60s, 150s and 300s at 10µA-intensity 3x/week. The ES therapy was not cytotoxic at any time of the experimentation. MSCs from ING presented higher percentage of cell viability at 60s of ES than MSCs from ABD stimulated for 150s and 300s. Moreover, MSCs from ING presented higher proliferation rate than ABD at all three ES time-application. The use of ES therapy during the osteogenic differentiation contributed positively to improve the mineralization process. MSCs from ING presented 30% greater mineralization at 150s, and 50% at 300s, while ABD showed 50% greater at 60s of ES. In conclusion, MSCs from ING and ABD are able to increase cell proliferation and improve osteogenic mineralization, but with different responses depending on the ES stimuli at the same experimental condition, which reinforce the needing for further investigation on the use of MSCs derived from fat tissue and ES to optimize their use for cell proliferation and mineralization in regenerative medicine.