Acne vulgaris is a common skin disease that affecting 85%-100% population. Acne vulgaris is a multifactorial disease affecting the pilosebaceous follicle. One of the factors that affecting acne vulgaris is face cleanliness. The purpose to keep face cleanliness is to decrease the presence of microorganism from the surface skin. This study was conducted to determine the relation between facial cleanliness with the incidence of acne vulgaris. This is an observational with cross sectional study and to analize data using both chi square and mann whitney methods. This samples in this study is a college student aged 18-22 years old. The results of bivariate analysis to mann whitney test obtained p = 0.022, thus there is a significant relation between the frequency of face washing with the incidence of acne vulgaris, while the results of the chi square test obtained p = 0.590, thus there is no significant relation between face washing after using cosmetics with the incidence of acne vulgaris.