Here we report the validation of a commercial real-time PCR method to detect vertebrate DNA in vegan food products. The method was tested on pure plant matrices of three different food categories: bakery and pastry products (chocolate biscuits), processed products (vegan burgers), sauces and vegetable drinks. Method specificity, sensitivity, and robustness were determined by amplifying the DNA extracted from target and un-target animal species diluted with the DNA extracted from the pure plant matrices to a final concentration of 0.01% (100 mg/kg). The method proved specific, resulting in the detection of all species at a limit of detection of 0.01%. Invertebrate species (insects, shellfish, crustaceans) were tested and no DNA detected. The method demonstrated selectivity and sensitivity in detecting vertebrate DNA. As such, it can be applied in the certification of genuine vegan products for human consumption.